Isolation and Quantification of Genomic DNA from Mycobacterium tuberculosis0

👤biogeny 🕔28-Oct-2017

Mycobacterium tuberculosis is an obligate pathogenic bacterial species in the family Mycobacteriaceae and the causative agent of tuberculosis. First discovered in 1882 by Robert Koch, M. tuberculosis has an unusual, waxy coating on its cell surface primarily due to the...

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Phenol/Chloroform Precipitation of DNA0

👤biogeny 🕔28-Oct-2017

The extraction of nucleic acids involves adding an equal volume of phenol-chloroform to an aqueous solution of lysed cells or homogenized tissue, mixing the two phases, and allowing the phases to separate by centrifugation

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PLANT DNA EXTRACTION0

👤biogeny 🕔28-Oct-2017

To extract DNA, tissue samples are taken from plants, and crushed to break open the cells. Next, a buffered salt water solution is added into which DNA dissolves easily. The DNA is purified by adding an organic solution into which...

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Isolation of Plasmid DNA0

👤biogeny 🕔28-Oct-2017

A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. Plasmids naturally exist in bacterial cells, and they also occur in some eukaryotes. Often, the genes carried in plasmids provide bacteria with genetic...

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